Figure 7
From: Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
Angiogenic stimulation via MV supplementation. (a) The inducible capacity of MVs for vascular tube formation. 3 μg/mL of IBE-MVs and 3D-MVs were added to HUVECs plated on Matrigel, and resulting tube formation was observed along with a control (basal medium) and VEGF-treated groups by microscopy. The size bars indicate 200 μm. (b) Loop numbers (c) branch numbers and (d) branch length values of the resulting tube formations were quantitatively compared. While HUVECs treated with IBE-MVs showed similar levels to the VEGF-treated group, 3D-MVs demonstrated a greater capacity to stimulate HUVEC tube formation with higher significance than the other groups. Data are presented as the mean ± SEM. Differences among groups were evaluated by one-way ANOVA at a level of significance of p < 0.001 (***), 0.001 < p < 0.01 (**), or 0.01 < p < 0.05 (*).
