Figure 1
Recording of circadian bioluminescence rhythms in primary SCN cultures prepared from ClockΔ19 mutant new-borns containing the PER2::LUC genetic modification. (A) Representative traces of PMT bioluminescence recordings of wild-type (+/+), heterozygous (+/clk), and homozygous (clk/clk) Clock mutant SCN. The vertical axis shows the bioluminescence intensity and the horizontal axis shows the number of hours of the PMT recording. (B–E) Characteristics of PER2::LUC bioluminescence rhythms in Clock mutant SCN cultures. (B) The period length of the bioluminescence rhythms, which are defined as the mean time of the peak-to-peak between the second and fifth waves. (C,D) The mean of the peak and trough values of the bioluminescence rhythms, respectively. (E) The mean bioluminescence intensity per cycle. Results were estimated using the second, third, and fourth waves. Means ± SEM, Tukey-Kramer test, *p < 0.05, **p < 0.01, ***p < 0.001.
