Figure 7
From: Proteomic evidences for microcystin-RR-induced toxicological alterations in mice liver
Effect of MCYST-RR on DNA fragmentation in mice liver. (A) agarose gel showing DNA fragmentation. M- Molecular weight marker (1 Kb DNA ladder), Lanes 1–4; 1- untreated control mice liver DNA, 2–200, 3–300, and 4–400 µg MCYST-RR kg body wt−1 treated mice liver DNA. Liver was excised from control and MCYST-RR treated mice (after 6 h) and DNA was isolated for fragmentation assay. (B) histogram showing changes in intensity along with % fragmentation of DNA of mice liver following treatment of varying concentrations of MCYST-RR. Value of % DNA fragmentation is proportional to the intensity of low molecular weight DNA. Equal amount of DNA from all the sets including control was loaded in each well. Test of DNA fragmentation was made in duplicate and repeated three times under identical conditions.
