Table 1 Details of protein spots identified by MALDI-TOF MS.

From: Proteomic evidences for microcystin-RR-induced toxicological alterations in mice liver

Spot No.

Accumulation (U/D)/Ratio to control*

Approx. PI/MW (kDa) on the gel

No. of peptides Matched

Mascot score of matching and significance level#

Sequence coverage (%)

Accession number

Predicted name of protein

1

U/3.1

5.2/50

4

67.9

56.1

gi|28875330

Immunoglobulin heavy chain VDJ region [Mus musculus]

2

U/2.0

4.5/75

9

71.6

24.8

gi|254540166

Glucose-regulated protein 78 kDa [Mus musculus]

3

D/0.43

5.5/32

11

83.2

35.1

gi|6677739

Regucalcin [Mus musculus]

4

U/3.4

6.0/70

7

70.4

18.8

gi|163310765

Serum albumin precursor [Mus musculus]

5

U/1.71

6.4/47

10

86.6

31.1

gi|309265190

Alpha-enolase-like isoform 2 [Mus musculus]

6

D/0.62

6.6/52

6

64.7

14.1

gi|148687772

Aldehyde dehydrogenase 2, mitochondrial, isoform CRA_a [Mus musculus]

7

U/1.8

9.0/30

14

113

59.6

gi|148670365

Aldolase 2, B isoform, isoform CRA_b [Mus musculus]

8

U/2.7

10/27

13

115

59.6

gi|6754084

Glutathione S-transferase Mu 1 [Mus musculus]

9

D/ 0.25

9.1/23

10

68.2

21.3

gi|4929247

Alpha-dystrobrevin 2a [Mus musculus]

10

U/2.2

6.3/26

8

65.6

23.2

gi|17921976

3-Hydroxyanthranilate 3,4-dioxygenase [Mus musculus]

11

D/0.21

7.8/23

7

66.3

12.9

gi|124487419

Keratin, type II cytoskeletal 2 epidermal [Mus musculus]

12

U/3.6

5.1/37

6

68.8

26.7

gi|55669634

Mouse glycine N-methyltransferase

13

U/2.03

9.0/58

16

135

29.4

gi|115704

RecName: Full = Catalase

14

U/4.2

9.0/15

7

103

78.9

gi|145301549

Hemoglobin α, adult chain 2 [Mus musculus]

15

D/0.44

7.8/42

11

86.1

31.7

gi|544273

RecName: Full = Fumarylacetoacetase

16

U/1.9

7.5/18

8

71.1

43.8

gi|309128

Carbonic anhydrase 2 [Mus musculus]

17

U/2.8

10/16

4

64.8

56.1

gi|28875330

Immunoglobulin heavy chain VDJ region [Mus musculus]

18

D/0.31

7.5/35

4

66

45.3

gi|148703101

mCG142516, isoform CRA_b [Mus musculus]

  1. U- Up-accumulated, D-down- accumulated. *Based on quantification of data generated by PDQuest analysis between control Vs treated (300 µg kg body wt−1). Highest expression (6.3 times) with 200 µg kg body wt−1 treatment for the hemoglobin alpha. #Significance level of matching was p < 0.05 for all the proteins.