Figure 2 | Scientific Reports

Figure 2

From: Myelination of Purkinje axons is critical for resilient synaptic transmission in the deep cerebellar nucleus

Figure 2

LES and LEHet DCN neurons display altered tonic inhibition and spontaneous AP firing. (A) Representative deep cerebellar nuclei (DCN) cells in LE, LEHet, and LES, which were filled with Alexa 568 during whole-cell recordings. (B–D) Whole-cell current clamp recordings of spontaneous APs at membrane potentials of −50, −55, −60, and −65 mV in DCN neurons of LE (B), LEHet (C), and LES (D) rats, demonstrating increased firing frequency in LES, and to a lesser extent, LEHet DCN neurons. (E–G) Spontaneous action potential firing in DCN neurons of LE (E), LEHet (F), and LES (G) rats at −50 mV in the presence of 10 μM bicuculline. (H) Summary of spontaneous firing frequency at various membrane potentials from −70 mV to −50 mV in LE, LEHet, and LES DCN cells. (I) The effect of bicuculline on spontaneous firing frequency (at −50 mV) in LE, LEHet, and LES DCN cells. *, **, and *** indicate p < 0.05, p < 0.01, and p < 0.001, respectively.

Back to article page