Figure 4
From: A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis
Analysis of drug-induced DSBs by the UDAR assay. (a,b) Universal Donorno cut and mCherry-expressing plasmid (1 μg:0.1 μg) were co-transfected into HeLa cells in 6-well plates. Eight hours after transfection, the cells were exposed to different doses of bleomycin for 30 min (a) or ICRF193 for 24 h (b). After 48 h, the cells were subjected to FACS analysis. Representative flow cytometry plots are presented. (c,d) Correlation between EGFP percentages and doses of bleomycin (Spearman’s correlation coefficient (R) = 0.92) (c) or ICRF193 (Spearman’s correlation coefficient (R) = 0.92) (d). The error bars indicate s.d. (n = 3).
