Figure 2 | Scientific Reports

Figure 2

From: Draxin regulates hippocampal neurogenesis in the postnatal dentate gyrus by inhibiting DCC-induced apoptosis

Figure 2

Increased apoptosis in draxin-deficient mice. (A) Apoptosis in the draxin wild-type (WT) and knockout (KO) DG detected by immunohistochemistry for single-strand DNA at E17.5, P0 and P15. (B) Quantification of ssDNA-immunoreactive cells in the entire DG at E17.5 and P0, and in the subgranular zone (SGZ) at P15. (C) Immunostaining of active caspase-3 on the WT and KO DG at E17.5, P0 and P2. (D) Quantification of AC-3-immunoreactive cells in (C). Black lines in (A and C) emphasize the pial surface of the DG. The boxed areas in (A and C) are magnified in the upper right of panels. Arrowheads indicate immunoreactive cells. The average number of immunoreactive cells was calculated from 5 independent brains per group. Error bars indicate s.e.m. *p < 0.05, **p < 0.01 against WT mice (Student’s t-test). CA: Cornu Ammonis. Scale bars, 200 µm.

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