Figure 4 | Scientific Reports

Figure 4

From: Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells

Figure 4The alternative text for this image may have been generated using AI.

Detection of apoptosis induced by M22 by flow cytometry and confocal microscopy. (A) Evaluation of apoptosis by Annexin V/PI dual staining assay. Numbers of Annexin V-and PI-positive cells were determined using flow cytometry. Quadrant 1: necrotic cells; Quadrant 2: late-apoptotic cells; Quadrant 3: viable cells; Quadrant 4: early apoptotic cells. (B) The percent of apoptotic cells are represented by bar diagram. (C) Morphologic changes of A549 cells treated by M22 for 48 h were fixed and stained with Hoechst 33258. M22 treated cells show apoptotic, condensed and fragmented fluorescent nuclei. Bars, 10 μm.

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