Figure 5 | Scientific Reports

Figure 5

From: Regulation of Signaling Pathways Involved in the Anti-proliferative and Apoptosis-inducing Effects of M22 against Non-small Cell Lung Adenocarcinoma A549 Cells

Figure 5The alternative text for this image may have been generated using AI.

Effect of M22 on intracellular ROS levels, DNA fragmentation and mitochondrial transmembrane permeability (MMP, ΔΨm) changes. A549 cells incubated with M22 at different concentrations for 48 h were stained with DCFH-DA, fluorescein isothiocyanate-dUTP and JC-1 dye. The signal were assessed from fluorescence by flow cytometry and determined in terms of mean fluorescence intensity (MFI). Dot plots show that (A) ROS and (C) DNA fragmentation levels increases in a concentration-dependent manner. Bar diagram show that (B) percentage of elevated ROS cells, ROS production and (D) percentage of cells with TUNEL expression, DNA fragmentation degree increases in a dose-dependent manner. (E) Dot plots show that ΔΨm decreases in a dose-dependent manner. (F) Bar diagram showing percentage of apoptotic and nonapoptotic cells are hown on the right side of each panel.

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