Figure 5
From: Effects of pulsing of light on the dentinogenesis of dental pulp stem cells in vitro
Dentinogenic differentiation of hDPSCs after 21 days of LPL daily treatment. 300 Hz PW-LPL with duty cycles 30% and 77 mJ/cm2 total energy density were applied. (A) Bright field images of hDPSCs stained with ALP staining kit at 21 days after 30%-3Hz-LPL daily irradiation. (B) ALP activity absorbance relative to control (n = 3 in each experiment and 3 replicates). (C) Western blots (W.B.) for ALP, DMP1, OPN, OCN, and BSP. (D) Bar graph showing mean density relative to beta-actin by analyzing Fig. 5C. The significance between controls and the LPL was all much lower than 0.05 (n = 3 in each experiment and 3 replicates).
