Figure 6
TFP promotes glioma cell proliferation in vivo. (A) Glioma cells were subcutaneously injected into nude mice (4 mice each group). The control group was injected with 0.9% sodium chloride and the treatment group was intraperitoneally injected with TFP (2 mg/kg, every 3 days) after the xenograft tumour formed. (B) Tumour sizes were measured every day using a Vernier calliper after the tumour became palpable. The tumour volumes were calculated as follows: volume = (D × d2)/2, where D indicated the longest diameter and d indicated the shortest diameter, plotted as the mean ± SEM. *P < 0.05. (C) Apoptosis, Ki67 and PCNA protein expression levels were examined using IHC in tumour tissues derived from the indicated mouse group (400×). (D) Immunohistochemical scoring and apoptosis scoring in indicated groups. Each bar represents the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.
