Figure 5

Mitochondrial content and degradation activity analyses (mitophagy) by confocal microscopy. (a) IMR90 cells stained with the mitochondrial marker MitoTracker red (MTr) and the mitophagy marker BNIP3L/Nix upon control, OR, GR and OGR. (b) Quantification of the MTr, BNIP3L/Nix as well as the BNIP3L/Nix to MTr ratio by measuring the total fluorescence. *p ≤ 0.05, **p ≤ 0.01. P-values were determined by one-way ANOVA with Dunnet’s multiple comparison’s test. Data represent mean ± s.e.m.