Figure 1 | Scientific Reports

Figure 1

From: Compositional Bias in Naïve and Chemically-modified Phage-Displayed Libraries uncovered by Paired-end Deep Sequencing

Figure 1

Analysis of diversity in naïve libraries. (a) The libraries were sequenced at every step of standard production of phage libraries: (i) synthesis of random oligonucleotide (“oligo”); (ii) extension and ligation into the vector; (iii) transformation into bacteria and expression of the library for 4.5 hours. After extension and ligation of synthesized oligonucleotides into the vector, the library was sequenced before the transformation into bacteria. (b) Synthetic NT-TriNuc library. (c) Primers used for amplifying ligated or naïve oligonucleotide DNA. (d) Generation of PCR product. Alignment of forward and reverse primers to 18-bp and 14-bp sequences flanking the variable region at the N-terminus of the pIII gene in M13KE vector, respectively.

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