Figure 3 | Scientific Reports

Figure 3

From: Compositional Bias in Naïve and Chemically-modified Phage-Displayed Libraries uncovered by Paired-end Deep Sequencing

Figure 3

Effect of PCR parameters on quality of deep sequencing. (a) Increasing the number of PCR cycles from 10 to 35 led to only subtle changes in percentage (0.5% to 0.7%) of forbidden codons (FC). (b) The percentage of FC did not change significantly when reads were filtered by quality scores, but the overall fraction of mapped reads decreased dramatically when over-stringent quality filtering was employed. (c) We observed minor drift in the relative ratio of each TriNuc codon in all four positions of the synthetic libraries of Ser-X-Cys-XXX-Cys peptides when the number of PCR cycles was increased from 10 to 35.

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