Figure 6

Phenotypic features of the CPAR2_303700Δ/Δ strain. (A) Growth kinetics of wild-type (CLIB 214), CPAR2_303700Δ/Δ and CPAR2_303700 reintegrant (RI) strains in YPD liquid medium, at 30 °C. (B) Growth on 10 mM H₂O₂ supplemented YPD medium, and (C) on YPD solid medium at different temperatures. (D) In silico 3D structure comparison of the hypothetical Cpar2_303700 and the previously described S. cerevisiae Cgi121 proteins. Scale bar: 10 ångström (Å). (E) 3D structure of the complex formed by Cpar2_303700 and Bud32 and its comparison to the Cgi121-Bud32 complex. Predicted H-bonds (bottom left) and the surrounding hydrophobic residues (bottom right) maintain the stability of the Cpar2_303700 – Bud32 complex. Moderately attenuated virulence of CPAR2_303700Δ/Δ (F) in vitro after J774.1 macrophage infection, following (G) G. mellonella larvae inoculation and (H) BALB/c mice infection. Statistical significance was determined by two-way ANOVA and Dunnett’s multiple comparisons tests in case of J774.1, unpaired Mann-Whitney tests in case of BALB/c mice infection and Mantel-Cox (Log-rank) test after G. mellonella infection: *p ≤ 0.05; ****p ≤ 0.0001.