Figure 3
tChIP-Seq from Camk2aH2B-FLAG showed accumulation and depletion of reads along known neuronal genes and non-neuronal genes, respectively. (A) Histogram of number of peaks along differential changes of single H3K4me3 ChIP-Seq and tChIP-Seq in Camk2aH2B-FLAG compared to those from RosaH2B-FLAG. Bin width is 0.1. (B–D) Distribution of reads from tChIP-Seq of RosaH2B-FLAG and Camk2aH2B-FLAG along each gene locus. RPM: reads per million. (E) Cumulative distribution of reads in peaks corresponding to neuron development and blood vessel development genes along differential changes between Camk2aH2B-FLAG and RosaH2B-FLAG tChIP-Seq. Significance is calculated by Mann-Whitney U test. (F) GO analysis along differential changes between Camk2aH2B-FLAG and RosaH2B-FLAG tChIP-Seq with iPAGE.
