Figure 3 | Scientific Reports

Figure 3

From: Combination Therapy Strategy of Quorum Quenching Enzyme and Quorum Sensing Inhibitor in Suppressing Multiple Quorum Sensing Pathways of P. aeruginosa

Figure 3

(A) Structure of G1. (B) Combination effects of G1 and AiiA on PAO1-lasB-gfp reporter strain. (C) Dose-response effects of G1 (50 µM) added with AiiA (32 µg/ml and 16 µg/ml) on lasB-gfp. (D) IC50 values of the single and combination treatments on lasB-gfp, G1 (1.36 ± 0.08 µM), AiiA (6.88 ± 0.46 µg/ml), G + A 32 µg/ml (4.45 ± 0.15 nM). Calculation was performed using GraphPad Prism 6 software, taken at the time point between 4–6 hours where the inhibition started to occur. (E) Dose-pair effects of G1 and AiiA on lasB-gfp. The concentration of DMSO and buffer correspond to the highest test concentration used in the assays. All experiments were done in triplicate manner, only representative data are shown.

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