Figure 1

Transcriptomics analysis of macrophages exposed to carbon-based nanomaterials. Global gene expression profiling of human monocyte-derived macrophages (HMDM) was conducted after 24 h exposure to single-walled carbon nanotubes (SWCNTs) or graphene oxide (GO) (see Supplementary Table 1). (a) Heatmap of differently expressed genes in response to 10 and 30 µg/mL GO (line 1 and 2, respectively) or 10 and 30 µg/mL SWCNTs (line 3 and 4, respectively). Upregulated transcripts are presented in red and downregulated transcripts in green (log2 fold change > 0.75). The genes affected upon exposure to SWCNTs (n = 52) and GO (n = 7) did not overlap. (b) Canonical pathways modulated in HMDM after exposure to SWCNTs (see Supplementary Table 2); ranking was performed according to multiple testing corrected p-value.