Table 1 Details regarding the plasmids used in this study.
Plasmids | Primer | Instruction |
|---|---|---|
pM3QCP | M-5UTR-F/M-5UTR-R, QCP-F/QCP-R, M-3UTR-F/M-3UTR-R | CP mutants and based on viral infectious cDNA clones. |
pQ3MCP | Q-5UTR-F/M-5UTR-R, MCP-F/MCP-R, Q-3UTR-F/Q-3UTR-R | |
pGBK-MCP | BD-MCP-F/BD-MCP-R | For YTH work and based on vector pGBKT7 and pGADT7 (Clontech). |
PGBK-QCP | BD-QCP-F/BD-QCP-R | |
pGAD-FdI | AD-FdI-F/AD-FdI-R | |
pGAD-FdI(1–55) | AD-FdI-F/AD-FdI-55R | |
pGAD-FdI(56–145) | AD-FdI-56F/AD-FdI-R | |
pMCP-YFPN | Bi-MCP-F/Bi-MCP-R | For BiFC assay and based on vector pSPYNE-35S and pSPYCE-35S36. |
pMCP-YFPC | ||
pQCP-YFPN | Bi-QCP-F/Bi-QCP-R | |
pQCP-YFPC | ||
pFdI-YFPN | Bi-FdI-F/Bi-FdI-R | |
pFdI-YFPC | ||
pFdI-GFP | Sub-FdI-F/Sub-FdI-R | For subcellular localization assay and based on modified vector pCAMBIA1300. |
pFdI(1–55)-GFP | Sub-FdI-F/Sub-FdI-55R | |
pTRV-FdI | TRV-FdI-F/TRV-FdI-R | For VIGS assay and based on TRV mediated VIGS system40. |