Table 1 Details regarding the plasmids used in this study.

From: Cucumber mosaic virus coat protein induces the development of chlorotic symptoms through interacting with the chloroplast ferredoxin I protein

Plasmids

Primer

Instruction

pM3QCP

M-5UTR-F/M-5UTR-R, QCP-F/QCP-R, M-3UTR-F/M-3UTR-R

CP mutants and based on viral infectious cDNA clones.

pQ3MCP

Q-5UTR-F/M-5UTR-R, MCP-F/MCP-R, Q-3UTR-F/Q-3UTR-R

pGBK-MCP

BD-MCP-F/BD-MCP-R

For YTH work and based on vector pGBKT7 and pGADT7 (Clontech).

PGBK-QCP

BD-QCP-F/BD-QCP-R

pGAD-FdI

AD-FdI-F/AD-FdI-R

pGAD-FdI(1–55)

AD-FdI-F/AD-FdI-55R

pGAD-FdI(56–145)

AD-FdI-56F/AD-FdI-R

pMCP-YFPN

Bi-MCP-F/Bi-MCP-R

For BiFC assay and based on vector pSPYNE-35S and pSPYCE-35S36.

pMCP-YFPC

pQCP-YFPN

Bi-QCP-F/Bi-QCP-R

pQCP-YFPC

pFdI-YFPN

Bi-FdI-F/Bi-FdI-R

pFdI-YFPC

pFdI-GFP

Sub-FdI-F/Sub-FdI-R

For subcellular localization assay and based on modified vector pCAMBIA1300.

pFdI(1–55)-GFP

Sub-FdI-F/Sub-FdI-55R

pTRV-FdI

TRV-FdI-F/TRV-FdI-R

For VIGS assay and based on TRV mediated VIGS system40.