Figure 5
From: Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies
mHtt IBs in transgenic R6/2 and homozygous HdhQ150 mouse models are ubiquitin positive. Confocal images of ubiquitin co-stained with mHtt IBs in (a) 14-week-old R6/2 mouse brain and (b) 22-month-old HdhQ150 mouse brain slices. Exemplary confocal pictures of mouse cortex tissue containing ubiquitin-positive aggregates, note that ubiquitin antibody staining is present in the core of the aggregate and correlates with the intensity of the Htt antibody staining. IBs were stained with an anti-Ub antibody P4D1 and anti-Htt antibody S829. Nuclei were stained with DAPI. Scale bar upper panel is 8 µm; lower panel is 0.25 µm.
