Figure 6 | Scientific Reports

Figure 6

From: Mechanical phenotyping of K562 cells by the Micropipette Aspiration Technique allows identifying mechanical changes induced by drugs

Figure 6The alternative text for this image may have been generated using AI.

Panel a refers the Apoptosis induction evaluated as fluorescence intensity of fluorescein isothiocyanate–Annexin V-staining in one representative experiment. The histogram plots show the staining peaks in the different conditions for the K562 cell line cultures (Control = untreated cells; T6-T48 = OTC incubation for 6 h, 9 h, 15 h, 24 h, 48 h, respectively; H2O2 = represents the positive control of apoptosis induction on K562(see Materials and methods). The percentages of the K562 cells labeled with Annexin V are reported in each histogram plots. The amount of the apoptosis induction is related to the shifting to the right of the peak on the x-axis; Panel b refers the bar graph data (mean (percentage) ± SD) of the 3 performed experiments based on Annexin V staining for apoptosis evaluation. Control represents the untreated cells and refers to the apoptosis background (growth medium without OTC); T6-T48 represent the apoptosis evaluation at the different incubation times of the K562 cell line in a growth medium with the addition of OTC. As positive control of apoptosis induction is reported the effect of H2O2 (see Materials and methods). Thestatistical significance of each incubation time vs the negative control (untreated cells) is indicated with asterisk (P ≤ 0.005, with Paired T Test).

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