Figure 6
Influence of disulfiram on A-beta depositions and behavior in AD model mice. (a) Deposition of APP-cleavage products derived from transgenic human APP was assessed in brain slices of mice (description of treatment: see Fig. 4) by staining with antibody 6E10. Two cortical regions (c), subiculum (s) and dentate gyrus (dg) were used for densitometric quantitation from two slices per animal and corrected by a same-size background area as indicated in the scheme (I: whole area; II: magnification of dentate gyrus). Values are given as mean + SEM (b), n = 6 for control and 5 for disulfiram-treatment). (c) In the morning of day three, nest-building performance of the animals was determined by a scoring and also by weighing material that was not incorporated in the nest (n ≥ 7 per group). (d) Learning capability was tested in the mice by a novel object recognition paradigm performed in a Y-maze (n = 8 per group). Discrimination index is presented as mean ± SEM. Statistical analysis: unpaired Student’s t-test (***p < 0.001; *p < 0.05).
