Figure 3
From: Viral highway to nucleus exposed by image correlation analyses
Image correlation analysis of capsid movement. (A) Average fluorescence intensity image showing the position and fluctuations of lamin C-EGFP. (B) Cytoplasmic capsid intensity trajectories along the scanned line in the cytoplasm, at the nuclear envelope (NE) and in the nucleoplasm as a function of time and distance are shown. (C) Autocorrelation function (ACF) analysis of intracellular viral movement. (D) Pair correlation function (pCF) analysis showing a positive correlation across the lamin C-EGFP and viral capsid transit over NE (arrowhead). Pseudocolor coloring with intensity increasing from blue to red demonstrates the distribution of fluorescence intensity. X axis represents distance in pixels along the line scan and y axis is logarithmic time scale. (E) File averaged diffusion coefficients from the ACF analysis of capsid movement in the cytoplasm, at the NE and in the nucleoplasm (38 measurements from 14 cells) are shown as boxplots. The error bars represent the ± standard error of mean (±SEM).
