Figure 4
From: Viral highway to nucleus exposed by image correlation analyses
Number and brightness analysis of capsid dynamics. (A) ACF based file averaged analysis showing the number of all moving particles in the cytoplasm (dark gray), at the NE (light gray) and in the nucleus (white) (n = 15 cells). Number (B,C) and brightness (D,E) analyses of fast and slow moving particles in the cytoplasm, NE and nucleus. The black horizontal lines represent the median, the crosses represent the mean, and boxes represent the quartiles of data range including the median. (F) A histogram of the average brightness of the slow fluorescent viral particles as a function of distance from the nuclear lamina in all cells studied. (G) Single-cell fluorescence intensity map showing the number (red) and brightness (yellow) of cytoplasmic and nuclear capsids and distribution of lamin C (green, time-average of 100 line scans). (H) Histogram showing absolute brightness distribution of fast nuclear viral particles.
