Figure 3
Growth characteristics of S. depilis-associated Zygosaccharomyces sp. (a) Pseudomycelium of Zygosaccharomyces sp. in S. depilis brood cells. (b) Cells of Zygosaccharomyces sp. SDBC30G1 cultured in 30G broth, pH 4.5. (c) Agar invasion of Zygosaccharomyces sp. SDBC30G1 colony (15GF agar medium, pH 4.5, 30 °C, after 10 days). (d) Pellicle formation of Zygosaccharomyces sp. SDBC30G1 (5 mL, 15GF broth, pH 4.5, static condition, 30 °C, inoculum OD600 0.1, 6-well plates, after 7 days). (e) Ascospores of Zygosaccharomyces sp. SDBC30G1 (30% glucose and 0.5% of yeast extract liquid medium, static condition, 30 °C, inoculum OD600 0.1, after 7 days).
