Figure 4
From: Prevention of Retinal Degeneration in a Rat Model of Smith-Lemli-Opitz Syndrome
Quantification of selected 7DHC-derived oxysterols in neural retina as a function of dietary regimen and postnatal (PN) age. Oxysterols were quantified by UHPLC-MS/MS as described in Methods; values given in ng/retina. (A) Oxysterol structures (see inset, panel B, for key): 4α-hydroxy-cholesta-5,7-dien-3β-ol (4α-OH-7DHC), 4β-hydroxy-cholesta-5,7-dien-3β-ol (4β-OH-7DHC), 7-ketocholesterol (7k-Chol), and 3β,5α-dihydroxycholest-7-en-6-one (DHCEO). Individual oxysterol levels at (B) PN 60 days and (C) PN 80 days. Total oxysterol levels at (D) PN 60 days and (E) PN 80 days. For PN 60 days rats, N = 4 for each group. For PN 80 days rats, Control group, N = 4; AY1 group, N = 2; AY2 group, N = 3; AY3 group, N = 3. Significance levels (Student’s t-test): *p < 0.05; **p < 0.005, and ***p < 0.001 for comparison of AY1 vs. AY2 and AY3 values; #p < 0.05 and ##p < 0.005 for comparison of AY2 vs. AY3 values.
