Figure 2

Cytokine exposure elevates basal islet [Ca²⁺]_i and disrupts GSCI. (a) Representative Fura-2 AM recordings (F₃₄₀/F₃₈₀) of changes in β-cell [Ca²⁺]_i for nontreated (gray) and cytokine treated (black) WT mouse islets (the lines above the figure indicate glucose concentrations), (b) average [Ca²⁺]_i oscillation frequency for nontreated (gray) and cytokine treated (black) WT mouse islets with 11 mM glucose (N ≥ 12 islets), (c) representative Fura-2 AM recordings of changes in β-cell [Ca²⁺]_i for nontreated (gray) and cytokine treated (black) TALK-1 KO mouse islets, (d) average [Ca²⁺]_i oscillation frequency for nontreated (gray) and cytokine treated (black) TALK-1 KO mouse islets with 11 mM glucose (N ≥ 30 islets), (e) average basal [Ca²⁺]_i AUC (0–200 sec) for nontreated (gray) and cytokine treated (black) WT and TALK-1 KO islets (N = 3 animals), (f) average 1^st phase GSCI AUC (200–600 sec) for nontreated (gray) and cytokine treated (black) WT and TALK-1 KO islets (N = 3 animals), and (g) average 2^nd phase GSCI AUC (600–1000 sec) for nontreated (gray) and cytokine treated (black) WT and TALK-1 KO islets (N = 3 animals). Statistical analysis was conducted using 1-way ANOVA and uncertainty is expressed as SEM (*P < 0.05, **P < 0.01, ***P < 0.001).