Table 1 Oligonucleotide primers used in this study.

From: Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L)

Name

Sequence

Position

C9ΔNLS1-MF

GACGATGACGATAAGATG/GACAAGAAGTACAGCATC

1305–1322/1371–1388 [1]

C9ΔNLS2-MF

CTCAGCTGGGAGGCGAC/TAAGAATTCCTAGAGCTC

5455–5471/5520–5537 [1]

gRp30-F

caccGCAAGGGTATACTGAACATC

125,144–125,163 (R) [2]

gRp30-R

aaacGATGTTCAGTATACCCTTGC

125,144–125,163 (R) [2]

X330gRR-F

ATGCTTACCGTAACTTGAAAG

181–201 [1]

X330gRR-R

ATTTGTCTGCAGAATTGGCG

405–424 (R) [1]

CP204L-PSF

CACAAGTTGTGTTTCATGC

125290–125308 (R) [2]

CP204L-PSR

TGAAGATCCACGGTTACCC

124670–124688 [2]

  1. Primers C9ΔNLS1-MR and C9ΔNLSR-MR were reverse complementary to the respective forward primers, and slashes (/) indicate the positions of the deleted NLS encoding sequences. Nucleotides printed in lower case have been added for convenient cloning of the hybridised oligonucleotides. Nucleotide positions refer to the forward or reverse (R) strand of the sequence of [1] pX330-U6-Chimeric_BB-CBh-hSpCas920, or of [2] ASFV Georgia34.
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