Figure 3
From: Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail
Chemical cross-linking of PABPC1 on poly(A). (a) SDS-PAGE analysis of PABPC1 after treatment with the cross-linking reagent, BS(PEG)9, in the presence or absence of A24, A48, A72, or long poly(A) ranging from 150–500 bases. (b–e) Chemical cross-linking of PABPC1 and RRM1/2/3/4 at each molar ratio of a 24-base unit in poly(A) over protein. SDS-PAGE analysis of the cross-linked PABPC1 (b) and RRM1/2/3/4 (d). The ratios of the monomer and the multimer at each molar ratio in PABPC1 and RRM1/2/3/4 are plotted in (c) and (e), respectively. The monomer and multimer ratios were calculated by dividing the band intensity of the monomer and the multimer by the total intensity.
