Figure 2

Three-dimensional reconstruction and MIP of slabs for liver and lung. Livers and lungs were harvested from Vecad^cre/tdTomato^{flox/stop/flox} transgenic mice (blood vessels, red channel) and were labeled with LYVE1 antibody (lymphatic vessels, cyan channel). Organs were cleared and imaged using a light-sheet fluorescent microscope. Imaging was done using dual side illumination and fused (single side illumination is presented in Supplementary Figure 1). Data sets of liver (a–c) and lung (d–f) are presented as 3D volumes. Maximal intensity projections (MIP) of slabs (liver: 30 slices/23.1 μm, lung: 3 slices/4.86 µm) show the structure of an hepatic lobule with its mid central vein (g) and lung lobules surrounded by vasculature(j). High-magnification of rotated images (g,i) show the continuity of blood and lymphatic vessels. Scale bar: MIPs - liver 50 μm, lung 100 μm; High magnification images – liver and lung 100 μm. Image dimensions: liver 1200 µm × 600 µm × 600 µm, lung 1200 µm × 1000 µm × 400 µm, XYZ (whereas the XY plane is perpendicular to the objective lens). Image dimensions of zoomed in, cropped and clipped images (c,g): liver 600 µm × 400 µm × 400 µm lung 600 µm × 400 µm × 400 µm, XYZ.