Figure 3
From: Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
Three-dimensional reconstruction and MIP of slabs of uterus and ovary. Uteri and ovaries were harvested from Vecadcre/tdTomatoflox/stop/flox transgenic mice (blood vessels, red channel) and were labeled with LYVE1 antibody (lymphatic vessels, cyan channel). Organs were cleared and imaged using a light-sheet fluorescent microscope. Tiles were stitched (10% overlap for stitching) creating large field of view stacks. Data sets of uterus (a–c) and ovary (d–f) are presented as 3D volumes. Maximal intensity projections (MIP) of slabs (Uterus: 33 slices/53.46 µm, ovary: 87 slices/140.94 µm) show the uterine spiral arteries, endometrium and myometrium as well as the ovarian follicles surrounded by capillaries. High-magnification of rotated images (g,i) show the continuity of blood and lymphatic vessels. Scale bar: MIPs - uterus 100 µm, ovary 200 µm; High magnification images – liver and lung 100 μm. Image dimensions: uterus 1200 µm × 1600 µm × 400 µm, ovary 3200 µm × 1600 µm × 1200 µm, XYZ (whereas the XY plane is perpendicular to the objective lens). Image dimensions of zoomed in, cropped and clipped images (c,g): uterus 900 µm × 800 µm × 200 µm, XYZ ovary 800 µm × 800 µm × 1000 µm.
