Figure 4
From: Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
Quantitative analysis of blood vessels in the ovary. A mask was created on the original data of ovarian blood vessels using Ilastik software. From that mask, filaments were calculated using Filament tracing module of Imaris software. A representative slice (a), the filament tracing (b), the mask created (c) and an overlay of the original data with the filaments (d) demonstrating high compatibility, are presented (scale bar 300 µm). A 3D reconstruction of the filaments, a mask of the whole ovary (e,g) and a selected single follicle (f) are shown. Numerical parameters calculated from the filament analysis are presented in Table 1.
