Figure 3 | Scientific Reports

Figure 3

From: Hyperbaric oxygen reduces inflammation, oxygenates injured muscle, and regenerates skeletal muscle via macrophage and satellite cell activation

Figure 3

HBO improved the early post-injury muscle environment and increased myofiber regeneration. (A) Representative image of the extracellular space (non-stained area) in H&E-stained sections. Scale bar: 100 µm (B) The ratio of the extracellular space per HPF was lower in the HBO group. n = 5. **P < 0.01 using Welch’s test. (C) The absorbance of Evans Blue in the supernatant from homogenized muscles was higher in the NT and HBO groups than in the intact group. The absorbance in the HBO group was lower than that in the NT group. n = 10. ***P < 0.001, *P < 0.05 using one-way ANOVA followed by Bonferroni post-tests. (D) Nucleus-centered myofibers (insets) were observed from 5 days after injury in the NT group. Regenerated myofibers (arrows) were counted. Scale bars: 100 µm (E) The number of regenerating myofibers in the HBO group was higher than that in the NT group at 5 days. n = 5. *P < 0.05 using Student’s t-test. Data are the mean ± SEM.

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