Figure 3
From: Differential effects of SUMO1 and SUMO3 on PKR activation and stability
Effect of inhibition of SUMOylation on PKR expression. (a) HeLa-wt, HeLa-SUMO1 and HeLa-SUMO3 cells were treated with 100 μM ginkgolic acid (GA) for 6 h. Equal amounts of cell extracts were revealed by Western blot using anti-His (left panel), anti-SUMO1 (middle panel) or anti-SUMO2/3 (right panel) and anti-Actin antibodies. (b/c) (b) Total cell extracts, (c) cytoplasmic (C) and nuclear (N) fractions from cells treated as in a, were analyzed by Western blot for PKR, Hsp90, Histone H3 or Actin. Uncropped images of blots are shown in Supplementary Figure 2.
