Figure 1
From: Defining the Akt1 interactome and its role in regulating the cell cycle
Standardization of Akt1 over-expression (A) and subsequent confirmation of expression by western blotting (B and C). (A) Standardization of Akt1 expression over a range of Tet concentrations (0.1–5.0 µg/ml); Lane M: Marker; Lane 1: Uninduced sample; Lanes 2–7 depict various Tet concentrations used-Lane 2: 0.1 µg/ml; Lane 3: 0.25 µg/ml; Lane 4: 0.5 µg/ml; Lane 5: 1.0 µg/ml; Lane 6: 2.5 µg/ml; Lane 7: 5.0 µg/ml. (B) Normal and Akt1 expressing HEK293 cells (in presence and absence of Tet) probed for Akt1 and HA probe, simultaneously; GAPDH was used as a loading control. Lane M: Marker; Lane 1: Lysate from normal HEK293 (minus Tet); Lane 2: Lysate from Akt1 expressing HEK293 (minus Tet); Lane 3: Lysate from Akt1 expressing HEK293 (+Tet). (C) Effect of Akt1 transfection on population doubling time.
