Figure 1

Subcellular relative distribution of APL^* species and effect of EGCG 100 μM pre-treatment from GP images. Generalized Polarization GP images of XY sections (at the surface of the coverslip; Z = 0) of representative groups of (a) HeLa-wt, (b) HeLa-APL-R cells, and (c) HeLa-wt and (d) HeLa-APL-R cells, pre-incubated with EGCG 100 μM for 30 minutes, all treated with different concentrations of added [APL^*] 5-10 nM, and [APL^*]/[APL] 10 nM/90 nM, and 10 nM/440 nM for t ∼ 20 minutes. Scale bar 10 μm. Dark green-white-blue violet color GP scale: Dark green and blue violet colors highlight cellular regions enriched in APL^*-B (polar) and APL^*-A (less polar) complexes, respectively. λ_exc = 750 nm. CH1: FF01 520/35; CH2: FF02 435/40, Dichroic filter: FF458-Di02. 1.2 ms/pixel.