Figure 5
From: Dynamic cellular maps of molecular species: Application to drug-target interactions
Quantification of fractional intensities of APL*-A complexes at CH2 and APL*-B complexes at CH1 from the FLIM phasor approach. Schematic illustration of the phasor plot corresponding to a single fluorophore in two different cellular microenvironments, (A and B), with single lifetimes τ A (red cursor) and τ B (blue cursor), τ A > τ B. Point P1 corresponds to a mix of molecular species A and B, plus the cell autofluorescence AF. Point P2 would correspond to mix of molecular species A and B, with lower fractional contribution of AF. Re-normalized fractional contributions of A and B determined from P1 and P2, excluding the AF contribution, are the same, and they will match the values directly obtained from the black point at the intersection of the line joining A and B phasors (linear combination of A and B species), corresponding to a mixture of A and B species and 0% AF.
