Figure 1 | Scientific Reports

Figure 1

From: SPK1-transfected UCMSC has better therapeutic activity than UCMSC in the treatment of experimental autoimmune encephalomyelitis model of Multiple sclerosis

Figure 1

The expression of SPK1 in transduced UCMSC in vitro. (A) Expression of GFP in UCMSC transfected by Ad-GFP as determined by fluorescence microscopy. (B) Flow cytometric analysis of GFP expression in UCMSC transfected by Ad-GFP. (C) Production of bioactive SPK1 in the supernatant of non-transduced (UCMSC) and transduced (UCMSC-SPK1). Supernatant from cultured cells was retrieved on the indicated time points, processed and analyzed using a mousespecific SPK1 ELISA assay. (D) Typical bands of SPK1 and GAPDH detected by western blotting. (E) Gray intensity analysis of SPK1. All data are presented as mean ± standard error of mean. (#P < 0.01, UCMSC-SPK1 vs UCMSC and UCMSC-GFP, $P < 0.01 UCMSC vs UCMSC-SPK1).

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