Figure 3
The observation of demyelination. (A) The white matter of the normal mice was dark blue, and the model group presented light blue. Demyelination was obviously improved in the UCMSC-SPK1 mice group as compared to that of UCMSC mice group. While compared to the normal mice, the myelinated fibers in UCMSC-SPK1 group were more sharpe (as shown by the arrow). (B) The demyelination score (#P < 0.01 the control vs the model group, *P < 0.05 the model group vs UCMSC group, **P < 0.01 the model group vs UCMSC-SPK1 group, @P < 0.05 UCMSC group vs UCMSC-SPK1 group). (C) There were a lot of myelinated fibers in the white matter of the normal mice, while sharply decreased in the model group (as shown by the arrow) under TEM. After UCMSC-SPK1 treatment, the myelinated fibers significantly increased. (D) The number of myelinated fibers in the ten fields were calculated and statistically analysed, the tendency was consistent with that of the demyelination score of LFB staining. (E,F) The determination of the Confocal, and the tendency was consistent with that of the demyelination score of LFB staining. And the MSCs labelled by red fluorescence were also observed in the spinal cords of the UCMSC and UCMSC-SPK1 groups (as shown by the arrow). (G,H) The results of WB also powerfully proved this phenomenon.
