Figure 5

Sertoli cell density is unaffected by exposure to phosphoramide mustard, cisplatin or doxorubicin. (A–D) Representative photomicrographs of cultured testis fragments showing immunohistochemical localisation of Sox9 (green) and BrdU (red), counterstained with DAPI (blue). (A) Control cultured testis contains seminiferous tubules lined with Sox9⁺ Sertoli cells. (B–D) Tissue from High concentration of each of: (B) PM, (C) CIS or (D) DOX shows similar expression patterns to Control, including the occurrence of proliferating Sertoli cells. White arrows indicate Sox9⁺/BrdU⁺ proliferating Sertoli cells. (E) Percentage of tubule containing Sox9⁺ Sertoli cells after exposure to increasing concentrations of: (Ei) PM, (Eii) CIS or (Eiii) DOX; n = 8 in all groups except Low and High PM, where n = 7. White scale bars represent 50 µm, yellow scale bars in inset represent 10 µm. Data are mean + SEM.