Table 1 Antibodies and conditions for immunohistochemistry.

From: Chemotherapy drugs cyclophosphamide, cisplatin and doxorubicin induce germ cell loss in an in vitro model of the prepubertal testis

Antibody

Catalogue#

Species

Dilution

Secondary Antibody

Visualisation

BrdU1

ab6326

Rat

1:500

GARat-b5; GARat-5946

Str-4885

CC32

Asp175

Rabbit

1:500

GAR-b5

Str-4885

COUPTFII3

PP-H7147-00

Mouse

1:200

GAM-5687

N/A

γH2AX (phospho S139)1

ab22551

Mouse

1:200

GAM-4888

N/A

Mvh1

ab27591

Mouse

1:100

GAM-5687

N/A

PLZF1

ab189849

Rabbit

1:3000

GAR-4887

N/A

Sox94

AB5535

Rabbit

1:500

GARat-5946

N/A

  1. Abbreviations:
  2. GAM-488: Alexafluor Goat anti-mouse IgG 488 nm.
  3. GAM-568: Alexafluor Goat anti-mouse IgG1 568 nm.
  4. GARat-594: Alexafluor Goat anti-rat IgG1 594 nm.
  5. GAR-488: Alexafluor Goat anti-rabbit IgG 488 nm.
  6. GAR-b: Goat anti-rabbit biotinylated.
  7. GARat-b: Goat anti-rat biotinylated.
  8. Companies: 1Abcam, UK; 2Cell Signalling Technology, USA; 3R&D systems, USA; 4Millipore, UK; 5Vector Laboratories, USA; 6Invitrogen, UK; 7Life Technologies, UK; 8Molecular Probes, Leiden, The Netherlands.
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