Figure 3 | Scientific Reports

Figure 3

From: 3D virtual histology of murine kidneys –high resolution visualization of pathological alterations by micro computed tomography

Figure 3

CT images of kidneys after in vivo PTA perfusion combined with ex vivo PTA staining. (A) Quantum CT scan of a kidney of a healthy mouse after in vivo PTA perfusion shows i) PTA filled blood vessels (arrows), bifurcated from the main renal vessels (not shown) to the arcuate vessels, and ii) the associated dense lattice of capillaries in the cortex in 3D (arrow heads). (B) In contrast, CT images of the same excised kidney after additional ex vivo PTA staining for 18 days visualizes the typical diffusion pattern, allowing the discrimination of anatomical structures such as cortex (c), medulla (m) and renal pelvis (rp). The vessel contrast is completely blurred. (C) An overlay of adjusted section planes of perfusion and diffusion data sets enables the co-localization of the blood vessels with the anatomical details of the kidneys. (D) 2D views of the same kidney at day 0 (kidney only perfused with PTA) and day 18 (kidney PTA perfused in vivo and PTA stained ex vivo) demonstrate the vessel contrast after PTA perfusion and the morphological contrast after PTA diffusion.

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