Figure 4 | Scientific Reports

Figure 4

From: Production of Wilson Disease Model Rabbits with Homology-Directed Precision Point Mutations in the ATP7B Gene Using the CRISPR/Cas9 System

Figure 4

Genotypes of the mutant pups. The target site of sgRNA1F is highlighted in red, and the PAM sequence is shown in green. The target site of sgRNA2R is shown inside the box, and the PAM sequence is labelled by a red wavy line. The point mutation is underlined in magenta, and the Bln I endonuclease cutting site is underlined in blue. The mutated base numbers and their sequences are shown on the right (+, insertion; −, deletion). A > G indicates that G was substituted by A, and T > G indicates that G was substituted by T. WT, wild type; KO, knock-out; KI, knock-in; SYNMUT, synonymous mutation.

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