Figure 2
From: The antidepressant fluoxetine acts on energy balance and leptin sensitivity via BDNF
FLX treated mice show enhanced response to acute leptin administration. (A) 14-h cumulative food intake in H2O-CFD and FLX-CFD mice at baseline and in response to i.p. leptin (3 mg/kg) injection. All data are expressed as % of the mean of the baseline of H2O-CFD. RM 2-way ANOVA (treatment x stimulus, F(1,45) = 7.93, P = 0.0072; matching, F(45,45) = 2.77, P = 0.0004; n = 22–25) followed by Bonferroni posthoc test, §§§ P< 0.001 versus baseline. (B) Left representative immunofluorescence showing expression of phosphorylated-STAT3 45′ minutes after an injection of saline (sal) or leptin (lep, 3 mg/kg) in the ARC of H2O-CFD (CNT) and FLX-CFD (FLX). Scale bar is 100 μm. Right, signal for pSTAT3 normalized to that of STAT3 was acquired for H2O- and FLX-CFD mice. All data are expressed as % of the mean of the H2O-CFD injected with saline. 2-way ANOVA (stimulus effect, F(1,34) = 77.18, P < 0.0001; treatment effect, F(1,34) = 14.06, P = 0.0007; n = 11–8, 10–9) followed by Bonferroni posthoc test, **P < 0.01 versus H2O-CFD, §§§ P< 0.001 versus saline. (C) OBRb mRNA value was determined by quantitative reverse transcription–PCR and standardized to TATA Binding Protein (TBP) in the hypothalamus of H2O-CFD and FLX-CFD mice. Student’s t-test (t = 3.212 df = 14, **P = 0.0063; n = 8). Data are presented as mean ± s.e.m.
