Figure 4

Evaluation of drift stability and resolution over time using nanorulers. (a) Sketch of a GATTA-PAINT HiRes 20 R nanoruler used for this experiment. (b) DNA-PAINT image of the nanorulers was acquired over a total time of 6 hours without the need of drift correction. (c) Localization transient of the white framed nanoruler in (b) indicating that the structure has been localized over the whole acquisition time without a detectable influence of drift. (d) Crossection of the white framed nanoruler in (b) including analysis of the spot widths by Gaussian fits.