Table 1 Example measurement uncertainty budget for the measurement of the mean nanoruler distance using setup no. 1.
Input quantity | Standard uncertainty of input quantity | Uncertainty contribution to \({\boldsymbol{u}}(\bar{{{\boldsymbol{a}}}_{{\boldsymbol{p}}{\boldsymbol{r}}{\boldsymbol{o}}{\boldsymbol{j}}}})\) in nm |
|---|---|---|
\(P\) | 0.5 nm/pixel | 0.3 |
\({d}_{k}\) | 0.05 pixel | 0.16 |
\({\delta d}_{{fit},k}\) | 0.0035 pixel | 0.01 |
\({\delta d}_{{env}}\) | 0.005 pixel | 0.5 |
\({\delta d}_{{blink}}\) | 0.001 pixel | 0.1 |
\({\delta d}_{{fp}}\) | 0.009 pixel | 0.9 |
\({\delta d}_{{model}}\) | 0.005 pixel | 0.5 |
Expanded measurement uncertainty (k = 2): U = 2.4 nm | ||
