Figure 6
From: Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes
Spectrophotometric assay of recombinant CysE and CysM protein variants. (A) Enzymatic activities of CysE recombinant protein variants: CysE (blue), CysE-C (orange), CysE-CM (yellow) are measured as a conversion of L-serine to CoA using Ellman’s reagent reaction at four time points (t = 5, 10, 15, and 20 minutes). (B) Enzymatic activities of CysM recombinant protein variants CysM (blue), CysM-C (orange), CysM-CM2 (open circle) are measured as a conversion of O-acetylserine to L-cysteine using Ninhydrin reaction at five time points (t = 0, 2.5, 5, 7.5 and 10 minutes). Protein negative control samples are represented as gray circle. Error bars represent the standard deviation as derived from the triplicates for each sample. Linear regression is presented to calculate the enzymatic activity of each protein variant.
