Figure 4 | Scientific Reports

Figure 4

From: Mitochondrial biogenesis and metabolic hyperactivation limits the application of MTT assay in the estimation of radiation induced growth inhibition

Figure 4

Radiation induces hyperactivation of mitochondria: (A) Photomicrographs showing mitochondrial membrane potential (MMP) in HeLa and MDA-MB-231 cells stained with TMRM (5 nM/ml; 30 min; 37 °C) at 24 hrs post irradiation. Radiation induced changes in mitochondrial MMP was quantified by DiOC6 (B) and superoxide radical was quantified using MitoSox (C) in HeLa and MDA-MB-231 cells using flow cytometer and presented as MFI fold change at indicated time points with respect to control. Star shows the statistical significance of change between the indicated groups. Data are expressed as mean ± SD from triplicates. *p < 0.05 vs. unirradiated control.

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