Figure 6
Role of Calcium in enhanced metabolic viability: (A) Cells were treated with A23187 (2 µM; 1 hr) to release ER stored Ca2+ for 4, 8 and 24 hrs and MTT index was measured by MTT assay in HeLa and MDA-MB-231 cells. The result is presented as fold change of metabolic viability/cell and normalized with respect to control. (B) Mitochondrial content was analyzed by flow cytometer using MitoTracker Green in Ruthenium Red (1 µM, continuous exposure) treated cells and presented as relative fold change with respect to control. (C and D) HeLa and MDA-MB-231 Cells were treated with Ca2+ chelator BAPTA-AM (20 µM for 2 hrs), and Ruthenium Red before irradiation and further metabolic viability was measured and presented as fold change difference with respect to control. Data are presented as mean ± SD from four independent experiments *p < 0.05 vs. control.
