Figure 3

Chia and pepsinogen level in the bovine stomach extract. Soluble protein fraction was prepared from bovine abomasum tissue in the absence of protease inhibitor and incubated at pH 7.6 or pH 2.0 for up to 60 min and analyzed by (a) SDS-PAGE, (b) total protein levels quantification, (c) chitinolytic and protease activity measured in bovine and pig stomach extracts at pH 2.0 as described in the Methods and (d) western blot using anti-Chia or anti-pepsin. The images of (a) were cropped from original full-length gel images with same exposure time shown in Supplementary Fig. S2a. The images of (d) were cropped from original full-length gel images shown in Supplementary Fig. S2b and c, respectively. The image of (d) Values in (b) and (c) represent mean ± SD from a single experiment conducted in triplicate. **p < 0.01.